Identification of Reactive Species Produced by Surfaces Dielectric Barrier Discharge Nonthermal Plasma with Gas Sources Variation (Air, N2, O2) to Kill Bacteria

Non-thermal plasma is one of the new techniques that being focused on in medical world, which used for sterilization because non-thermal known to have a bactericidal effect. Reactive species produced by are antimicrobial. One factor affecting species' reactive composition gas source used. Therefore, this study aimed determine effect from generation SDBD using different sources, namely free air, oxygen, and nitrogen, treating Escherichia Coli bacteria. The flow 1 liter/minute. were characterized Optical Emission Spectrometry (OES). treatment bacteria samples was repeated three times each treatment, then Anova test performed. results indicate bacterial death at decontamination level depends during treatment. Plasma an O2 has more effective inactivation ability, 6 x 107 CFU/ml, compared control, 409 CFU/ml. At same time, with air nitrogen sources 6.33 CFU/ml 41.67 These ROS RNS influences inactivation, where inactivating than